You are a researcher working in a molecular laboratory. One day, you need to run a batch of PCRs. You assembled the reagents including Toq polymerase, made the reaction mixtures, and ran them in a thermocycler. After the PCR program ran to completion, you performed a gel electrophoresis to check the quality of the PCR product. To your dismay, all reactions appeared to have failed! Nothing showed up on the gel. You reviewed the thermocycler’s log, which displayed the following information about your recent PCR cycles: Denaturation: 89 ”C for 1 minute Annealing: 50 “C for 40 seconds Elongation: 65 “C for 3 minutes

You are a researcher working in a molecular laboratory. One day, you need to run a
batch of PCRs. You assembled the reagents including Toq polymerase, made the reaction mixtures, and ran them in a thermocycler. After the PCR program ran to completion, you performed a gel electrophoresis to check the quality of the PCR product. To your dismay, all reactions appeared to have
failed! Nothing showed up on the gel. You reviewed the thermocycler’s log, which displayed the following information
about your recent PCR cycles: Denaturation: 89 ”C for 1 minute
Annealing: 50 “C for 40 seconds
Elongation: 65 “C for 3 minutes

 
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